产品参数

简要概述

产品基本信息

货号：17015

产品名称：生物素-11-dGTP

规格：25 nmoles

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：898.71

溶剂：水

产品介绍

生物素修饰的脱氧鸟苷5'-三磷酸酯广泛用于各种非放射性DNA标记反应，包括切口平移，随机引物标记，cDNA标记和3'-末端标记。可以通过切口平移，随机引物，3'-末端末端标记或PCR的方法将生物素11-dGTP酶促地掺入DNA中。数字“ 11”是dGTP与生物素之间连接子骨架中的碳原子数。连接子越长，生物素与抗生物素蛋白的相互作用越有效。另一方面，接头越短，dGTP就越有效地掺入DNA。建议链接器'11'的长度对于大多数应用而言是很好的。 Biotin-11-dGTP用于在各种杂交应用中产生生物素化的DNA探针，包括Southern印迹，Northern印迹，斑点印迹，固定细胞和组织。它在化学上等同于PerkinElmer（PE）的NEL541001EA。

参考文献

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