Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate with stable light output for mid-femtogram level detection for western blot analysis.
SuperSignal West Dura Extended Duration Substrate characteristics include:
• Sensitivity: mid-femtogram
• Stability: 24-hr working solution stability; 1-year kit stability at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 24 hours
• Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 µg/mL)
• Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)
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SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled CCD camera-based detection systems. Unlike substrates with signals that decline to barely detectable levels in 30–60 minutes, the signal produced with SuperSignal West Dura chemiluminescent substrate is stable for 24 hours, allowing multiple film or camera exposures.
Recommended Antibody Concentrations: | 2°: 1:50-250K (4-20ng/mL), 1°: 1:5K (0.02- 1µg/mL) |
---|---|
Signal Duration: | 24 hr |
Detection Method: | Chemiluminescent |
Product Line: | SuperSignal™ |
Product Size: | 100 mL |
Sensitivity: | Mid-femtogram |
Substrate Properties: | Chemical Substrate |
Substrate Type: | HRP (Horseradish Peroxidase) Substrate |
Sufficient For: 12 mini-gel blots; 1000 cm2 of membrane
• Luminol/Enhancer, 50 mL
• Stable Peroxide Buffer, 50 mL
Store at room temperature.
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